them being seniors..it's that they are male.
At about 18 months his hormones have kicked in and the girls(who are intact) are HIS!!!!! He is not willing to "share" with the other boys.
No matter what you try ..in the end ..keeping them apart is all that is going to work.
When my younger male turned 18 mths. he went after my 6 year old male. From that day on they could never be out together..it's been 18 mths..amd we play "musical" dogs with them.
I have tried a number of things..nothing has worked.
Also it has nothing to do with wheither you are Alpha. I have a pack of 13 most of the time..this pack number and dynamic changes as the rescues come and go. No one else fights and I have control of the pack. BUT.. Bubba hates Tank..and that's all there is to it.

http://www.mts.net/~bittybul/
If you breed....rescue

except Duncan was neutered at age 8 mths. he is now 28 mth old. our old guy a boxer is 12, he was neutered at 1 year of age. So neutering does not help. The fighting started sometime last spring and continues to happen more frequently had to break them up 3 time last Sat. we decieded that day to start spearating them when we are home. you know one in for a couple hrs, then the other one. We had to put a stop to letting them both sleep with us quite some time ago. As they would get into it in the middle of the night on top of the bed!!! they are fine when alone.
I always thought my boyz would be best of friends to the end

To my opinion there is a packorder problem between the seniors and the oldies. Your Bulldog doesn't have a problem with other dogs, so it is personal. Neutering won't change that, but you could give chemical neutering a try. With chemical neutering your dog gets an jab with hormons, which mimic the neutered state. It will last for about 4 weeks, and you'll can assess if actual neutering will help your problem. It is worth a try.

With 17 months he is getting adult. He has been getting away with it for months, but now he has passed a border and those oldies won't take it anymore.

You've got 2ptions: let it work out with the two oldies. Take them out in pairs (1 Mastiff, 1 Bulldog) to a place that both dogs don't know and take them off leash. Start walking and keep walking and let them work it out.
The other option is to get the Bulldog under control, and call him back each time he is eyeing the old guys. Control him! If you can't control him, keep him separated. You are alpha.

Good luck!

I had tried that prior but will give it a shot again. I think a combination of Isaac maturing and no longer allowed the puppy status by the older boys and Isaac's normal I want to be boss personality is coming to a head. While the mastiffs are old they are not feeble and that little man I think believes he is a mastiff in size.

The crazy thing is he is so incredibly loving with the other two Mastiffs but both are girls and being intact. Isaac is a show dog but if neutering him would curb the problems I would do it in a heart beat but I've not seen the theory pan out with neutering vs not neutering unless it is done at a younger age than Isaac.

Any thoughts on this idea?

Just an idea..

You could try putting him on a long leash/lead and letting him near them (10 ft or so). If he starts to get agrresive pull on the leash and verbally repremand him. He is probably just trying to stake his claim as a tough guy and this is how he thinks he can do it.